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Consequently, we performed a phylogenetically independent contrasts (PICs) analysis (Felsenstein 1985; Pagel 1992).
The COMPARE 4.6b was applied to PICs analysis (Martins 2004).
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The PIC analysis was conducted using the computer program Compare 4.6b on the transformed variables [70].
We found similar trends when phylogenetic correlation was taken into account, but as our focus was on interactions and ANCOVAs with environmental factors, which cannot be accommodated by a PIC analysis, we performed all of our regression analyses directly on the species data.
PIC analysis produced similar results to that of PGLS.
For PIC analysis, the molecular topology with branch lengths was imported into Mesquite 2.75 [ 51].
PIC analysis was conducted for each ecological category with ecology treated as a binary state.
PIC analysis revealed several additional significant correlative relationships within individual families (Additional file 4).
PIC analysis of the fungal genome data showed GH32 gene abundance was significantly correlated with ecological strategy.
PIC analysis of biomolecular complexes remains a challenging endeavor, but one that can reward the investigator with otherwise inaccessible information.
The raw data were not normally distributed; therefore, in order to linearize the data for PIC analysis [ 70], the mean measurements for DNA amount were log transformed.
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