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Worms were then cleaned twice by washing in M9 buffer (5 g l−1 NaCl, 3 g l−1 KH2PO4, 6 g l−1 Na2HPO4, 1 m m MgSO4) for 10 min each before picking onto lifespan plates.
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For video capture, worms were picked onto conditioning or control plates and left for 6 h.
Sections were picked onto Superfrost Plus or Apes coated Superfrost slides.
White colonies where picked onto fresh agar plates containing ampicillin and grown overnight at 37°C.
15 30 animals at the L4 stage were picked onto each plate and subsequently transferred to newly seeded plates every 1 2 days.
For the preconditioned food race, L4 worms were picked onto the conditioning plates (50 worms per plate) and left for the conditioning period (2 to 48 h).
Sections were picked onto 200 mesh copper grids, stained with uranyl acetate and lead citrate and examined under a Tecnai Twin (FEI) electron microscope.
In both cases L4 stage animals were picked onto fresh plates seeded with food and allowed to develop at 20°C, and one-day old adults were analyzed.
To assay the body bends, nematodes were picked onto a second plate and scored for the number of body bends in an interval of 20 sec.
They were then individually washed for 2 min in M9 to remove residual ethanol and placed onto a non-food plate for 1 min to remove excess liquid, before being picked onto a food race plate, 2 cm from the edge opposite the food and left for 5 min. After 5 min a 30 second video was recorded of the behaviour of the worm.
Rolling F1 animals were picked onto single plates to generate transgenic lines.
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