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All NOESY peaks were picked using the peak-picking mode in Sparky (http://www.cgl.ucsf.edu/home/sparky) for individual strips with noise and artefact peaks removed manually.
The harvest itself is also a horror story, on top of the environmental devastation, this is cotton picked using forced labour.
Colonies were picked using sterile cloning rings and expanded to form sub lines.
Protein spots representing greater than twofold alteration in expression were picked using an Ettan Spot Picker (GE Healthcare Biosciences).
After incubation under 30 °C for 4 days, each colony was picked using inoculating loop and then transferred to another plate by streaking for further purification and growth.
Operational Taxonomic Unit (OTUs) were picked using de novo OTU picking protocol with a 97% similarity threshold and singletons and doubletons were discarded in the QIIME software.
632 sub-volumes with a box size of 84 pixels were picked using IMOD (Kremer et al., 1996) and extracted using RELION1.4 (Bharat et al., 2015).
The resulting transmitted waveforms were picked using a customized Graphic User Interface (GUI) and then corrected for zero-time and column shell delays.
The best agent is picked using a set of rules from a rule base, which is currently maintained manually by an expert.
Single bacterial colonies, positively identified as containing the respective plasmids from white/blue screening, were picked using sterile pipette tips, cultured in 5 mL Luria Bertani (LB) broth (Difco™, USA) containing 5 μL of 100 μg/mL ampicillin (Biobasic, Canada).
These were picked using computer-generated random numbers.
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