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Single blastomeres of 4- to 8-cell embryos were isolated and picked up under the microscope for PCR amplification and Sanger sequencing.
At each round, 4 6 single plaques were picked under fluorescent microscope.
Plaques with a fluorescent signal can be identified in OFTu cells after 12 h pi, and individual green plaques were picked under fluorescent microscope at 36 h pi.
Stable BioT-REST expressing cells were selected in 2 µg/ml puromycin and individual clones were hand picked under a microscope.
NeuN+ (neuronal) and NeuN /Ki67– (glial) nuclei were isolated via FACS from brain tissue, individually picked under microscope and subjected to linear WGA.
Fluorescent colonies were identified and picked under an epifluorescence microscope (Nikon SMZ1500).
Quickly, intact and well isolated fibers were picked under stereo-microscope and washed first in DMEM and then in phosphate buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.76 mM KH2PO4, pH 7.4).
For hqEx168 and hqEx174, because the transgene arrays were not chromosomally integrated, GFP positive worms were hand-picked under the microscope and pooled to make lysates.
Stage 10 11 embryos (5 and 7.5 hours after egg laying) from a cross between D r72 /TM3, twi-GAL4 UAS-Gfp D r513 /TM3, twi-GAL4 UAS-Gfp D r513hand picked under a fluorescence dissecting microscope.
Relatively large forsterite (or olivine) crystals were recovered by picking under an optical microscope.
Individual cells (n = 30 from each colony) were hand-picked under a light microscope using a heat-elongated glass micro-pipette.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com