Exact(1)
Using this distribution, we can compute the probability of a randomly picked insert having a specific size.
Similar(59)
To evaluate the method of prediction, a subset of miRNA candidates were subjected to experimental validation: A total of 17 candidates were randomly picked, inserted along with approximately 250 nt flanking genomic sequence on either side into an intron of an eGFP expression plasmid (pJEBB, Figure S3), and overexpressed individually in HEK293 cells.
Plasmid minipreps were processed from randomly picked insert-positive colonies using the GeneJET Plasmid Miniprep Kit (Fermentas).
Plasmid minipreps were processed from randomly picked insert-positive colonies (10 for each biological situation) using the GeneJET Plasmid Miniprep Kit (Fermentas).
If the quality was satisfactory, 96 single clones were picked and insert analysis was performed as with the clone pools.
White colonies were picked for insert screening using PCR with M13F and M13R primers, and then grouped by RFLP with restriction enzymes RsaI and/or MspI.
In each case, eight resulting clones were picked, the inserts sequenced and subsequently allocated by the ARB software package.
PCR products were isolated using Qiagen columns, then cloned into the Promega Easy-T vector and transformed using competent NEB5α from NEB. Single colonies were picked and inserts amplified using colony PCR and Clontech Advantage.
I [Flanagan] used a prepared bank of questions from which I could pick and/or insert some of my own questions.
Bake for 30 to 40 minutes, until a wooden pick inserted into the center comes out clean.
Bake 60 minutes until wooden pick inserted in center comes out clean.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com