Exact(15)
G418-resistant ES cell colonies were picked individually at day 10 of selection (0.4 mg/ml G418) and clones with a targeted Pink1 gene were identified by PCR with primer P1 (5'-attgctgaagagcttggcggcgaatgggct-3') located in the neo gene and primer P2 (5'-tgc tgactgctgcaagagccaggcgatca-3') located in Pink1 exon 8 adjacent to the 3' end of the targeting vector (shown in Figure 1).
On the other hand, if in the second screen, animals are plated on a small number of plates, but picked individually to those plates, instead of being loaded from a liquid culture, then γ≈1, and the efficiencies of the first and second screens are 0.145 and 0.133, respectively.
Animals resembling Caenorhabditis were picked individually onto 55 mm culture plates.
F1 rollers were picked individually to plates and allowed to lay eggs for 1 day.
The clones (20 colonies) were picked individually and amplified in 3 ml of LB medium at 37°C overnight.
The clones were picked individually and amplified in 10 mL of Luria-Bertani (LB) medium at 37°C overnight.
Similar(45)
Sadly, only a select few stations will be available to pick individually at launch.
If you find that picking individually through Europe's real estate stocks is too taxing, let a pro do it for you: Henderson Global Investors runs the London-quoted TR Property Investment Trust and the Luxembourg-based Horizon Pan European Property Equities Fund.
When shopping, pick individually-sized foods, rather than foods that come in large containers.
Picked out individually, however, almost every picture is comical.
At present, a whole mass of nanotubes is produced and the ones that show the right device-like behaviour are picked out individually.
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