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Three colonies from each transformant were picked for growth curve analysis.
For each tolerance test, about 50 single colonies were then picked for growth assay in challenging medium.
After about 2 weeks, the stable clones were picked for growth on plates, and maintained in 450 μg/ml G418 for the detection of ideal clones.
The cells were allowed to grow in the presence of G418 for two weeks, and clones were picked for growth on plates to confluence.
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Colonies were robotically visualized and counted, and single isolates from each sector were picked for inoculation into 1mL growth media (LB/antibiotic) using a customized Megapix robot (Genetix), and 96-well culture blocks were grown overnight at 37°C in the presence of appropriate antibiotic.
were picked for maturity analysis.
Growth on MRS was observed for 21 of the 105 analysed samples, and one or more colonies were selected and picked for each of the 21 samples.
But it kept a buy rating and 670p target: Serco remains one of our European business services sector picks for its cyclical resilience, organic growth re-acceleration and exposure to austerity-based outsourcing.
Investors pay him management fees for picking growth stocks, not for buying Treasury bills.
For the unselected populations we picked a single colony for growth without screening.
For the 22 monomorphic populations (we began with 24 populations but two had to be discarded due to contamination), we picked a single colony for growth and screening.
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