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Multilayered transformed G418-resistant colonies were picked for further analysis and no transformed foci were observed in G418 selected vector transfected control cells.
Approximately 70 particles could be identified within a single 1K × 1K micrograph, and in total 20236 and 18659 particles were picked for further analysis (see Experimental section).
Cells were selected in media containing 400 μg/ml geneticin (G-418; Invitrogen) for 3 weeks, and individual stable clones were picked for further analysis.
Positive entry clones were picked for further analysis by colony PCR with M13 forward (5'- TGTandACGACGGCCAGT-3') and M13 reverse (5'- CAGGAAACAGCTATGACC-3') primers.
The isolates were transferred frozen to the Mycology Laboratory of the Wadsworth Center in Albany, New York, USA, where they were streaked on Niger seed agar plates (3 ) to check for purity and reconfirmation of their identity; a typical colony was picked for further analysis.
Similar(55)
The top ten features of cortical thickness with highest MI were picked first for further analysis.
Finally, genes that are selected in common for DNA methylation and genotypes were picked up for further analysis.
One of them was picked for further studies.
Colonies lacking PCR products were picked for further verification.
Colonies with more intense fluorescence were picked for further investigation.
A minimum of 2 or 3 plaques were picked for further plaque purification.
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