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Colonies of 20 potential RQ7/pDH10 transformants were picked for further analyses.
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Those genes overlapping with CNVRs completely or partially were considered as copy number variable and picked out for further analyses.
One of them was picked for further studies.
Colonies lacking PCR products were picked for further verification.
Colonies with more intense fluorescence were picked for further investigation.
A minimum of 2 or 3 plaques were picked for further plaque purification.
One to 3 presumptive E. coli colonies on CHROMagar™ ECC were picked for further confirmation.
From this survey, 7 promising peptone candidates were picked for further kinetic investigations.
Positive entry clones were picked for further analysis by colony PCR with M13 forward (5'- TGTandACGACGGCCAGT-3') and M13 reverse (5'- CAGGAAACAGCTATGACC-3') primers.
After selection, drug-resistant colonies were manually picked for further expansion and screening by genomic PCR and Southern blot.
Both infected and non-infected fragments were crushed and individual CFU's were picked and transferred to fresh MA plates for further analyses and DNA extraction, as previously described.
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