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Colonies were grown in ES medium and those exhibiting relatively homogeneous epithelium morphology were considered as promising epithelial-like cells [16] and picked for further expansion and downstream study.
Four to six colonies were tested for basal β-galactosidase activity and one picked for further transformation with the prey vector (with the insert and without any insert as control).
One of them was picked for further studies.
Colonies lacking PCR products were picked for further verification.
Colonies with more intense fluorescence were picked for further investigation.
One to 3 presumptive E. coli colonies on CHROMagar™ ECC were picked for further confirmation.
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Furthermore, for many mutagenesis schemes, only healthy F1 animals are picked for subsequent screening, further reducing the number of animals producing low brood counts.
ESC-like colonies were visible under these conditions and emerged as early as 7 21 days post-nucleofection, and manually picked for expansion and further characterization.
The top ten features of cortical thickness with highest MI were picked first for further analysis.
It was not picked up for further episodes.
As many as 50 of these could be picked out for further processing in the Discrimination and Control Computer (DCC), which ran more tests on those tracks and assigned each one a probability of being the warhead or decoy.
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CEO of Professional Science Editing for Scientists @ prosciediting.com