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Therefore the observed influence of pIC, temperature and time on PKR mRNA expression could impact the fish's ability to fight a viral infection.
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At 6HPI, both the microarray and the QPCR studies showed an significant up-regulation of IκBα mRNA by pIC at both temperatures (Table 1 and Figure 9A).
These genes responded significantly to pIC at both temperatures and time points, although the magnitude of the fold up-regulation was variable.
At 6HPI, ISG15 showed the highest response to pIC at both temperatures (20.77-fold 20.77-foldd 134.8-fold at 10°C), and RSAD2 (alias viperin) showed the highest differential mRNA expression between fish injected with pIC at the two temperatures (134.8-fold 134.8-fold16°C vs. 10°C).
To use it, you snap a photo of yourself after you've pieced together a good outfit, and then the app will automatically tag the pic with the current temperature.
For each time point (e.g. 6HPI) a two-way ANOVA with injection (i.e. pIC or PBS) and temperature (10°C or 16°C) as factors was then carried out.
Both clustering results show a clear distinction (with a few outliers) between the groups of fish injected with pIC at the different temperatures and time points.
GO term enrichment analysis was performed to compare the pIC response at different temperatures using Fisher's exact test as implemented by GOSSIP in the Blast2GO software with a p-value cutoff of <0.01.
Biological process GO mapping results for the genes differentially expressed between fish injected with pIC at the different temperatures (i.e. 10 vs. 16°C) were normalized to GO term hierarchy level 2 and plotted as pie charts.
In addition, clustering based on the 339 genes differentially expressed between fish injected with pIC at the different temperatures and sampled at 24HPI, indicates that the mRNA expression profile of pIC-injected fish held at 16°C and sampled 6HPI was most similar to that of fish injected with pIC at 10°C and sampled at 24HPI.
Several biological processes were highly represented (i.e. by more than 10 sequences) in the 629 genes differentially expressed between fish stimulated with pIC at the different temperatures (i.e. identified in the direct comparisons between fish injected with pIC at 10 and 16°C and sampled at 6HPI and 24HPI).
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