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The percent inhibition (PI) value was determined using the formula: PI =100 – (OD [test sample] / OD [buffer control] × 100).
The buffer materials of lower PI value could decrease swelling potential and increase permeability.
pI value was estimated to be 5.2 through two-dimensional electrophoresis.
PgAFP showed a cationic character with an estimated pI value of 9.22.
It had a molecular mass of 105 kDa and a pI value of 5.0.
The 297 bp E. nipponicum stefin sequence encodes a 98 amino-acid protein with a predicted molecular weight of 10.8 kDa and a pI value of 6.02.
The statistical analysis of the data showed optimum combination of diagnostic sensitivity and specificity at 98.8% and 99.2% respectively, with cut-off percentage inhibition (PI) value of >45.
Moreover, with diesel and biodiesels, the ηc increased to a maximum at 190 bar but declined at the higher Pi value.
We also designed alternative peptide-tags composed of plural aspartic acid residues (D5, D10 and D15-tags) to decrease the apparent pI value of the fusion protein.
The calculated PI value ranged between 22.88 and 61.65 (Additional file 1: Table S3).
All the postclassifiers are settled at PI value of more than 90% at QV of 20.
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