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The phytochemicals' effect on MscL function was measured using a calcein fluorescence assay.
To quantify the phytochemicals' effect to the bilayer deformation energy the potential of mean force (PMF) of dragging a large object (radius 0.9 nm) across a CG POPC bilayer was determined (see details in Supporting Information).
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We cataloged the phytochemicals' effects on membranes where they localize in the bilayer and what properties they alter.
As further evidence for the generality of the phytochemicals' effects, each membrane protein's response characteristics were similar for multiple phytochemicals.
Detailed descriptions of the phytochemicals' effects at the singe-channel level can be found regarding capsaicin, curcumin, EGCG genistein, and resveratrol (Supporting Information Figure S4).
We explored the phytochemicals' effects on KV2.1 channels heterologously expressed in the plasma membrane of CHO-K1 cells using voltage-clamp electrophysiology.
The authors speculated that the known protective effects of coffee (relaxing effect, antioxidant effect, phytochemical effects) might outweigh the risks of increased gastric acid secretion [80].
The phytochemical effects on endogenous NaV were determined by whole-cell electrophysiology in neuronal ND7/23 cells.
Most of the study primarily concentrated on individual phytochemical effects and their potential effects on the growth inhibition and prevention of cancer; however, the importance of these individual phytochemical might be difficult to confirm [ 39].
The aim of the present study was to evaluate the phytochemical effects, wound healing capacity and cytotoxicity of Arrabidaea chica, as well to evaluate the action of the crude extract and fractions of these plants on promastigotes of L. amazonensis.
To develop a consistent description of the phytochemicals' membrane effects, we used coarse-grained (CG) MD simulations based on the Martini force field to characterize how the five phytochemicals modify the structure and dynamics of CG 1-palmitoyl-2-oleoyl sn-glycero-3-phosphocholine (POPC) bilayers, at a 1 10 phytochemical:POPC molar ratio.
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