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The retention of antibacterial activity in aqueous leachates after removal of mineral particles suggests that the mechanism of antibacterial activity is dependent on soluble molecules and ions released from the minerals upon hydration, rather than physical shearing or electrostatic interactions between mineral and bacterial surfaces.
The Covaris S2 system was used for physical shearing.
This bias was observed with libraries prepared using both enzymatic and physical shearing (Additional file 2: Figure S1).
The CTAB isolation procedure produced genomic DNA fragments in the 40 kb size range, evidently a consequence of random, procedure-induced physical shearing.
After physical shearing, the DNA was blunt-end repaired and gel-fractionated to a fragment size of approximately 40 kbp as described by the manufacturer.
Cost assumption analysis suggested that the MspJI digestion method would be less expensive than the previously described cost-reduced physical shearing method (Additional file 10) [ 22].
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The prevention of biofouling resulted from both physical (shear forces) and biological (quorum quenching) effects of RMCF, and it was seen that RMCF could sustain its QQ effect over long-term operations.
The spurious velocities also induce a higher but non-physical shear stress during the droplet formation process, resulting in droplets forming prematurely with shorter lengths.
RCA, CB and WR in particular are found to also meet the physical and shear strength requirements for aggregates in pavement base/subbase applications.
A comprehensive laboratory evaluation of physical and shear strength characteristics of recycled C&D materials was undertaken using gradation, Los Angeles Abrasion, unconfined compression, California Bearing Ratio (CBR), direct shear and consolidated drained triaxial tests.
Experimentally determined gradation, physical and shear strength characteristics as well as electrochemical properties of ISFS used in the present study satisfied the standard specifications for a structural fill of different Guidelines/Codes of Practice.
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