Sentence examples for phosphatase detection from inspiring English sources

ALP activity was detected using an alkaline phosphatase detection kit (Sigma-Aldrich).

To examine the expression of cell-surface markers on cultured marmoset iPS cells, we detected alkaline phosphatase using the Alkaline Phosphatase Detection kit (Millipore) according to the manufacturer's instructions.

After thorough washing with PBS, antibodies specific for human MMP-9 (clone MAB911; R&D Systems, Minneapolis, MN, USA) and human MMP-2 (clone 75-7F7; OnCambridgeaMAridge, MA, USA) were applied, and primary antibody binding was detected using the alkaline phosphatase-antialkaline phosphatase detection system (Dako, Hamburg, Germany).

Cells were characterised by assay of alkaline phosphatase, detection of type 1 collagen, and production of osteocalcin.

Immunoblots were developed separately with antibody raised against Dm-AMP1 and Rs-AFP2, using the alkaline phosphatase detection method (Bangalore Genei, Bangalore, India).

AP staining was performed using an alkaline phosphatase detection kit (Millipore).

A streptavidin-biotin-alcaline phosphatase complex detection system was used for amplification and detection (Vector blue AP substrate kit III, Vector Laboratories, Burlingame, USA).

Sequential staining with CD68 detected macrophage infiltrate using an alkaline phosphatase linked detection signal.

Specifically, Melan-A, glial fibrillary acidic protein (GFAP), HMB45, and CD14 were detected using the UltraView Universal DAB Detection Kit, and the other antibodies were detected using the UltraView Universal Alkaline Phosphatase Red Detection Kit from Ventana (Tucson, AZ).

The ON-OFF fluorescence (FL) principle that the FL intensity of the N-CQDs was quenched in the presence of Fe3+ was further extended to the ON-OFF-ON and ON-OFF-ON-OFF ones for pyrophosphate anions (PPi) and alkaline phosphatase (ALP) detection, respectively, based on the specific competitive interaction of N-CQDs with Fe3+ and PPi and the hydrolysis of PPi in the presence ALP.

The fixed tissues were paraffin-embedded, sectioned (10 micron thickness) and stained with biotinylated anti-mouse IgG and developed with alkaline phosphatase ABC detection kit (Vector Laboratories).