Sentence examples for phosphatase data from inspiring English sources
Cells in growth medium expressed OP and alkaline phosphatase (data not shown) gene expression, suggesting these genes are not exclusively expressed by osteoblasts.
To test if the slowest-migrating band represents a phosphorylated form of AGS3, we immunoprecipitated endogenous AGS3 using our AGS3 antibody, incubated the immunoprecipitated AGS3 in the presence of Antarctic phosphatase (Figure 4A) or Calf Intestine Phosphatase (data not shown), and analyzed AGS3 migration on SDS-PAGE by western blotting.
Expression of SSEA-1 (supplementary material Fig. S1A) and alkaline phosphatase (data not shown) in different clones was analyzed by ES cell characterization kit (Millipore, Billerica, MA).
The intestinal inflammatory marker Alpl [ 18] was prominently upregulated, unlike Alpi and Alpi2, the intestinal isoforms of alkaline phosphatase (data not shown).
Porcine iPSCs cultured with a feeder-free system, Matrigel coated plates, and stem cell medium supplemented with 20 ng/mL LIF were positive for alkaline phosphatase (data not shown).
In all, 98±1.3% of cells were identified as AECII by the presence of dark blue inclusions, as revealed by modified Papanicolaou staining, and 93±2.1% of cells were positive for alkaline phosphatase (data not shown).
Further, the dephosphorylated status of the lower Bim EL band was established when protein lysates isolated from cells exposed to the different hormones were subjected to calf-intestinal-phosphatase (data not shown) or λ-phosphatase.
Interestingly, HNF1α suppression by siRNA in primary rat hepatocytes markedly decreased the SHP-1 level, but did not significantly affect the expression of other five phosphatases (data not shown).
Similar negative results were obtained using the catalytic subunit of protein phosphatase-1 (data not shown).
Ws-9 rES cells were also negative for alkaline phosphatase activity (data not shown) and TRA-1-60 (FigureS1G1G).
However, these cells were negative for alkaline phosphatase activity (data not shown) and TRA-1-60 (Figure 1I).
