The long-term anchorage-dependent assay was not performed in HCC1599 as it is a suspension cell line and thus the number of additional phenotypic assays is limited.
In this study, we found, by genome-wide transcriptional profiling and phenotypic assays, that YY1 overexpression prevents cardiomyogenic differentiation and maintains the proliferative capacity of CPCs.
For example, raw and derived gene-expression data might best be deposited in ArrayExpress or GEO; however, the results of a principal component analysis, control qPCRs or blots, or associated phenotypic assays could be stored in a generalist repository.
This genotyping scheme could be applied in microbiology laboratories as an alternative or complementary method to the traditional phenotypic assays, providing data for epidemiological studies.
There is an increasing need for viable lymphocytes in performing phenotypic assays for biomarker studies.
The choice of screen libraries such as scale, type, or format can be determined based on biological questions or the phenotypic assays developed.
DZ performed the phenotypic assays, microarray experiments, QPCR and RNAi.
Phenotypic assays for hemolysin activity, serum resistance, and biofilm formation were also performed.
LC performed the adaptations, sequencing, the majority of the phenotypic assays, and helped draft the manuscript.
Results: Using diverse phenotypic assays, we defined bioactivity for multiple compound libraries.
Claudia Hemsley helped design phenotypic assays, provided technical assistance and discussed results.
