The aim of this study was to verify the feasibility of phenols quantification coupled to 2-D electrophoretic protein analysis in rhizomes, as a novel "diagnostic" tool to monitor Posidonia meadows conservation status.
For phenolic compounds quantification, powdered samples (1 mL) were mixed with Folin and Ciocalteu's phenol reagent (1 mL).
The formation of blue compounds derived from FC-reactive phenols was independent of the structures of the phenolic compounds, enabling quantification of the full complement of phenolics from plant extracts.
However, 2-naphthol (quantification standard) and deuterated phenol (D6-phenol as a measure for loss of volatile phenol) standards were added to the substrates prior to separation by C18 non-endcapped SPE.
Separation, analysis and quantification of phenols were done in a high performance liquid chromatograph (HPLC) (Shimadzu LC-20 AT, Shimadzu Corporation, Japan), coupled with a Vydac C18 column (218 TP, 250 × 4.6 mm, 5 μm of particle size, Sigma-Aldrich, St . Louis MO, USA) and a SPD-10A UV VIS detector using the method of Lin et al. [33].
After the two fractions were obtained, we extracted the DNA using a standard phenol-chloroform protocol [24]; quantification was performed using GeneQuant Pro (Pharmacia), which showed variable concentrations varying between 10 to 100 ng/µl.
An 80% functionalization of the triphosphine-liposomes, based on carbohydrate quantification using phenol-sulfuric acid, was obtained, based on the assumption that about 40% of the triphosphine moiety is in the outer leaflet on the liposomes [ 47].
To the best of our knowledge, this is the first systematic screening for the quantification of phenols and antioxidant activity of the crude extract, fractions and compounds from A. adianthifolia.
In this paper a experimental design is applied to optimize the quantification of hindered phenol Irganox 1076, phosphite antioxidant Irgafos 168 and their oxidized product tri[2,4-di-tert-butylphenyl]phosphate from low-density polyethylene (LDPE).
