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Twenty-four siteses corresponding to the 12 disulfide bonds in the PDLP5 crystallographic dimer were located with the program SHELXD98, site-refinement and phasing was done in SHARP99, and the starting phases were used for automated model building in BUCCANEER100 and ARP/wARP101.
Heavy atom refinement and phasing was done with SHARP and resulted in an overall FOM of 0.51 for MAD and 0.75 for combined SIR and MAD.
Crystallographic phasing was done with SOLVE (19).
Phasing was done using DagPhase [ 26], accounting for both family structure and population linkage disequilibrium.
Initial phasing was done by molecular replacement (Phaser(49) module) using the structure of unliganded cocaine esterase (PDB code 3I2J).
(D – F ) As an additional test of model bias, phasing was done with a poly-alanine homology search model of lysozyme.
Similar(54)
Genotype quality control, imputation and phasing were done within each population.
The separation of the two phases was done by centrifugation.
A randomized crossover design study with two phases was done in the volunteer study.
Efficient extraction of dyes into IL phase was done within 1 min.
Lastly, our fourth experiment presents promising preliminary VT&R localization results, where the teaching phase was done during the day and the repeating phase was done at night.
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