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Results were binned per cell population in different phases of cell cycle (represented as % of total cell population).
CDK8, 3/cyclin C biosynthetic pathway plays a specific role in G0/G1/S phases of cell cycle.
Instead, incubation with copanlisib for up to 72 h caused a clear, dose- and time-dependent G1 cell cycle arrest, along with a corresponding decrease of the S and G2/M phases of cell cycle (Fig. 2e g).
Through the diverse array of substrates that are modified, SUMOylation impacts many cellular processes including various phases of cell cycle progression8,9,10, cellular differentiation11, heterochromatin formation,12 and the DNA damage response13.
c-ETS1 and c-ETS2 were recruited to the MCM4 origin respectively during the S and G1 phases of cell cycle.
Cell-cycle analysis by flow cytometry was done to determine the percentage of cells in different phases of cell cycle using a FACS Calibur BD Biosciencess, USA) instrument.
The cell cycle is a key factor in unregulated cell proliferation leading to cancer and differential sensitivity to apoptosis is linked to the distinct phases of cell cycle (Pucci et al. 2000).
The classification of cells into various phases of cell cycle was measured by flow cytometry technique, and the corresponding proliferation index and apoptosis index were calculated, as shown in Table 2.
Analysis of nuclear DNA by flow cytometry is interesting in fundamental research and has broadly contributed to improved knowledge on cell DNA content and their distribution in the various phases of cell cycle.
Cell populations in different phases of cell cycle were determined.
Phases of cell cycle were analyzed using flow cytometry as described previously [59].
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