These include the partition coefficient (KE or KP), which is calculated as the ratio of the enzyme activity or protein concentration in the top phase divided by the correspondent value in the bottom phase.
The plateau fraction (pf), which is defined to be the relative time spent in the active phase divided by the total burst interval [37], is another important parameter.
The rate of insulin release is believed to be roughly proportional to the "plateau fraction", which is defined to be the relative time spent in the active phase divided by the total burst interval [37].
The phase shift is the angular phase, expressed in degrees, while the delay is the angular phase divided by the frequency f NREM-REM and converted to minutes.
Partitioning is quantified in terms of the partitioning coefficient, K, which equals the concentration of solute in the top phase divided by its concentration in the bottom phase.
The K value was expressed as the peak area of the target compound in the upper phase divided by that of the lower phase.
Individual limb movements and interlimb coordination were calculated as follows: Inverse of stride duration x displacement of single limb during swing phase divided by swing duration.
The stock of hosts had been amplified as a large batch, harvested during the exponential growth phase, divided into individual tubes, and stored at -80°C.
