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Therefore, the pH of the mobile phase was adjusted to 7 (Fig. 3).
The pH of the aqueous phase was adjusted to pH 2.7 with orthophosphoric acid solution (10%).
pH of water phase was adjusted to 2.4 with 85% orthophosphoric acid and than methanol was added.
Interestingly very good separation was achieved on a C18 stationary phase with the mobile phase in which the pH of the aqueous phase was adjusted to 2.7.
The ratio of ACN to aqueous phase was adjusted to 1: 1, as increasing ACN led to bad separation and overlapped peaks.
The pH of the mobile phase was adjusted to pH 4.0 using orthophosphoric acid and the flow rate was 1 mL/min.
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To do so, the phase is adjusted when a pulse is received.
The distribution coefficient, logD, takes into account both the compound's non-ionized and ionized forms and in the determination of logD the aqueous phase is adjusted to a specific pH.
Yeast cells in the early log phase were adjusted to an OD600 of 1 with PBS.
In situations where distracting information can be anticipated, alpha power and phase are adjusted in order to 'close the door' of the visual systems just on time.
Cultures of strains in mid-exponential phase were adjusted to OD600 = 1.0 and 2 ml cultures were labeled with 100 µCi of [P] PO4 3 and cells allowed to grow for 3 hr.
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