PCR parameters were first optimized, and then reactions were performed allowing for product detection within the linear phase of mRNA transcript amplification for each primer pair.
Based on the phase of SSIII transcript(cluster33), it would not be surprising to find a circadian regulation of starch content in Ostreoccocus like in Chlamydomonas.
Phase of Arabidopsis transcript expression from the DIURNAL web tool [ 71], where phase is determined with HAYSTACK [ 35]. "nc" indicates noncycling transcript with a waveform assigned a PCC < 0.7 by HAYSTACK analysis.
Circadian-regulated transcription factors should confer the complete array of phased rhythms of transcript accumulation that is observed [ 8, 10].
We fit a type of generalized linear model (GLM) for the phase of gene transcripts with the three scores as predictors.
To begin to understand the relation between the core oscillator and the AtGRP7 slave oscillator and their respective downstream transcripts, we monitored recently published datasets for the phase distribution of transcripts controlled by TOC1 and LHY with reference to the Edwards dataset [ 35, 44, 45].
While regulatory functions for the newly discovered sRNAs have yet to be shown, the observation that many show growth phase-dependent regulation of transcript abundance is consistent with these sRNAs potentially regulating expression in a growth phase-dependent manner.
Interestingly we obsereved that at later phase of whitefly infestation, transcript of amino acid metabolic pathways of glycine, serine, threonine, alanine, aspartate, glutamate, valine, leucine, isoleucine and histidine were enriched (Table 5).
A functional category analysis was carried out for the transcripts differentially expressed in the acute phase of MI (77 transcripts common to both analyses: admission versus 6 months after AMI and admission versus control).
In addition to the remarkable consistency of expression phases of gene transcripts in tissues in which they are significantly circadian, transcripts of candidate genes were enriched for peaks at dusk (CT11) suggestive of E-box regulation.
