Sentence examples for phase of purification from inspiring English sources

A second phase of purification was conducted similarly using a TALON column (∼40 mL run at a rate of 0.3 mL/min) in buffer B [20 mM Tris-HCl and 300 mM NaCl (pH 8.0)] with 5 mM imidazole for column equilibration, 10 mM imidazole for washing, and a 200 mL gradient from 10 to 200 mM imidazole for elution.

Table 5 summarizes in detail the optimal methods for all phases of purification.

(A) Phase contrast pictures of purification of motor neuron cultures using Optiprep density centrifugation.

This study has systematically examined all phases of immunoglobulins purification, from sampling to detection and quantitation, has compared different methods and has considered each step in detail, with the aim of setting a specific, standardized and reproducible method to investigate mucosal antibodies and especially IgA.

Either preserving procedures, chemical addition and other phases of immunoglobulins purification did not affect the avidity and specificity of antibody binding; indeed, immunoglobulins from HIV-positive specimens conserved their anti-HIV reactivity when assayed in commercial ELISA kits (data not shown).

In the 2nd phase of the study, purification and characterization of the chitinase enzyme produced extracellularly by using Bacillus subtilis TV-125a bacteria, which was found to have the highest chitinase activity, were aimed; and in the last phase of the study, its antifungal activity was investigated against F. culmorum.

Target compounds 4c and 4d were obtained as white solids after concentration of the organic phase and purification of the residue by flash chromatography.

Glycosphingolipds (GSL) 2 (OCH9), 3 (OCH12) and 4 (OCH15) were obtained as white solids after concentration of the organic phase and purification of the residue by flash chromatography.

Target compound 1 was obtained as a white solid after concentration of the organic phase and purification of the residue by flash chromatography.

Reverse phase chromatographic purification of the foam derived extract revealed the presence of three main compound groups as represented in Fig. 2a.

After reverse phase HPLC purification of the proteins in the second HIC absorbance peak, a protein collected from the fraction in a major absorbance peak at the retention time of 35.4 min showed high lysozyme activity and was therefore designated cv-lysozyme 3. A total of 0.45 mg of cv-lysozyme 3 was eventually purified.