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She made the floor plan as difficult as she could imagine, opening with a half-pass in trot that moved into a half-pass in passage, followed by a combined piaffe and pirouette and straight into another phase of passage.
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Comprehensive reviews of the research literature through the initial phase of the passage of living wage laws were provided in Adams and Neumark (2004a and 2004b).
The time courses of growth of the isolates and lines used in the study were established and all parasites harvested for analyses from the same time point of late-log growth phase of early-passage cultures.
After RNA extraction from fibroblast clones in the exponential growth phase of similar passages (P12 18), microarray gene expression analyses were performed using Affymetrix GeneChip HT HG-U219 platesatray plathe at the Microarray Facility Tuebingen (MTF Services, Medical Genetics, Tuebingen, Germany).
All experiments were carried out on asynchronous log-phase cell cultures of passage 22-24.
In this study, we have demonstrated that specific changes in the DNA-methylation pattern arise already during the exponential phase of culture expansion (between passage 5 and passage 10).
Parasites were maintained in logarithmic phase of growth by passages each 2-3 dalwayslwaystartingng the cultures with 5 × 10 parasites/mL.
For some, a "metal phase" is merely a teenage rite of passage, much like bad hair and subsisting entirely on pint cans of Monster Energy.
Correspondingly, specific attention in this phase was paid to the points of passage between the global and local networks.
Next we measured the replication times of these deletion plasmids, using the same methods as in Figure 4. Flow cytometry showed that the rate of passage through S phase was almost identical for each of the four strains containing deleted plasmids.
Normally the cells reach a phase of crisis within 10 passages.
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