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The liquid phase of nitrogen has more potential for leaching.
The other physiological parameters measured, i.e, water content, dry matter and indicators of photosynthesis performance (chlorophyll fluorescence yield and content) were not significantly impacted by N depletion, indicating that the study was focused on an early phase of nitrogen deficiency.
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For a better understanding of the culture dynamics and for a suitable description of the bacteriocin production system, an unstructured mathematical model based on the two phases of nitrogen consumption was developed.
Zr Sn Nb specimens were implanted by nitrogen ions with a dose range from 5 × 1016 to 4 × 1017 ions/cm2 and then oxidized in air at 500 °C for 120 min. Glancing angle X-ray diffraction (GAXRD) was employed to examine the phase formation of nitrogen ion-implanted samples and phase transformation in the oxide films.
The buffy coat (400 μl) was transferred in a cryovial, immediately frozen in vapour phase of liquid nitrogen, and shipped in nitrogen dry shippers to the laboratory.
We used EDTA tubes to collect 7 mL whole blood that was promptly centrifuged on site at 2,500 rpm for 15 min. The buffy coat (400 μL) was transferred in a cryovial, immediately frozen in vapor phase of liquid nitrogen, and shipped in nitrogen dry shippers to the laboratory.
The sperm pellet was then resuspended in 300 μl of NIM medium, pipeted vigorously (to aid in removing the tails from the sperm heads), and 15 μl aliquots were distributed into cryovials and refrozen by placing and storing the vials in the vapor phase of liquid nitrogen in a liquid nitrogen freezer.
Since bubbles are generated due to quench under a fault condition, the phase change of nitrogen from liquid to gas occurs.
Bikaverin as well as gibberellic acid production started after 24 h when the culture entered the stationary phase because of nitrogen exhaustion and continued to increase till end of the fermentation.
Tissue was frozen in liquid nitrogen-cooled isopentane and stored in the vapour phase of liquid nitrogen.
PBMCs were transferred to the vapor phase of liquid nitrogen and stored until shipment on dry ice to European laboratories (2 20 h transfer time) or shipment in liquid nitrogen shippers for the four US laboratories (32 56 h transfer time).
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