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Lipid content was estimated at zero time and after 12 days (exponential phase) of cultivation.
The results obtained indicated that these products were absent or at markedly low levels when cells were subjected to the steady-state growth phase of cultivation under nitrogen depletion for the overproduction of bioplastics.
However, when the glucose concentration in the media became much lower for the parental and wild type strains than for the Δgox mutants in the later phase of cultivation due to the accumulation of gluconic acid, the pattern of production of organic acid in the parental and wild type strains started varying from the Δgox mutants.
Each phase of cultivation has a unique transcriptional and proteomic signature.
In our experiments, stimulation with peptides exhibited in a significant proliferative effect on the osteoblast-like cells, especially within the early phase of cultivation.
The production strategies were compared at 22 hours during the mid-logarithmic growth phase and after 27 hours at the late-logarithmic growth phase of cultivation.
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Samples were withdrawn in the exponential (light gray bars), early stationary (gray bars) and stationary (dark gray bars) growth phases of cultivation and were analyzed by gas chromatography.
Together with the amount of fatty acid unsaturation changes, these findings suggested that the fatty acid compositions were tightly regulated in the fungus during different phases of cultivation.
The claim is that computational approach taken after the gathering and assembling the transcriptome profiles (based on RNA-seq) for three rice landraces with two different systems of cultivation (two biological replicates per field type – rainfed and irrigated) in two phases of cultivation (dry and wet), allows establishing conclusions about interacting abiotic stimuli in field environments.
During the exponential growth phase of batch cultivations or continuous cultivations this method can be used to evaluate possible intracellular flux distributions based on measurements of exchange fluxes.
Moreover, the reduction of 1,3PD concentration in the later phase of the cultivation period could also occur due to a dilution effect (Figures 3 and 4). Figure 3 Fed-batch profile of Shimwellia blattae Sb6BP under different cultivation conditions in BM.
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