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The slow-cycling cells are predominantly in G2 phase of cell cycle.
Interestingly, the slow-cycling cells were paused in G2 phase of cell cycle and not in G0 as in the case of mammalian cells.
We used the Watson pragmatic algorithm (Supplementary Code 6) to obtain probability distributions for the cells being in G1, S and G2/M phase of cell cycle.
Accordingly, we proposed that the dosage of dFdC could determine the arrested phase of cell cycle, in turn affecting the recovery of dNTPs pools.
Fenofibrate suppressed the PDGF-BB-inducible progression through G0/G1 to S phase of cell cycle.
In metazoans, DNA replication is a highly regulated and ordered process that occurs during the S phase of cell cycle.
In addition, flow cytometry analysis on DU-145 cells revealed that these compounds arrest G2/M phase of cell cycle.
In addition flow cytometry analysis showed that both complexes also effectively arrest cells at G2/M phase of cell cycle.
Flow-cytometry analysis revealed that the compounds arrested the cells in G2/M phase of cell cycle.
Together, this data suggests that downregulation of NAP1L3 causes a growth arrest of UCB HSCs in the G0 phase of cell cycle progression and triggers apoptosis, which might explain the observed reduction in HSC numbers and activities.
NAP1L3 downregulation in UCB HSCs causes an arrest in the G0 phase of cell cycle progression and induces gene expression signatures that significantly correlate with downregulation of gene sets involved in cell cycle regulation, including E2F and MYC target genes.
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