This begins in the optimization phase of the assay: In test runs with a small number of compound plates, the assay has to possess a sufficient signal window (e.g., Z-factor [ 15]), stability, and sensitivity (e.g., measured by the effects of known control compounds) [ 16, 17].
Each test and injection phase of the assay was recorded for 2000 s.
The procedure of cell culture and treatment of nanoparticles was similar with the above phase of MTT assay.
During the evaluation phase of the assay, amplicons were analyzed by 2.0% agarose gel electrophoresis and, where necessary purified and sequenced to confirm their identity.
Since soil microbes grow at varying rates and produce secondary metabolites at varying times, and because their isolation media is often different from our E. coli competition media, we decoupled the isolate growth and compound production phase of the assay from the resistant-sensitive competition phase.
In this instance, all radioactivities remained in the aqueous phase of the assay work-up (data not shown).
The first phase (the preliminary equilibrium phase) of the assay (II) involves 7 reactions (Reactions 1,2,4,5,7,8,14), which are expressed as 12 ODEs (see Additional file 1: ModelEquations.doc).doc
The binding phase of the assay was performed at 37°C in a 5%% CO2 atmosphere for 15 min for THP-1 cells.
As noted, potent replicating-active compounds may register as potential nonreplicating-active compounds by killing in the outgrowth phase of the assay.
To inhibit the adhesion mediated by integrin α2 an integrin α2 binding antibody (Dianova, A.1.43) was added during the adhesion phase of the assay.
