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During log phase, nutrients are metabolised at maximum speed until one of the nutrients is depleted and starts limiting growth.
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In the depletion phase, nutrient solutions were exchanged twice a week for both the control and experimental plants.
Giving the support, e.g. cotton wool and fabric, for microorganisms to adsorb may ensure the nature physical separation between microorganisms and the liquid phase containing nutrients and products.
Iturin A was largely produced after the exponential growth phase when nutrients were near deficient [ 10].
This is reminiscent of yeast that transition into stationary phase when nutrients are exhausted.
Even in a planktonic phase, sufficient nutrients could be available in the water to sustain essential metabolic processes [ 6], albeit slowly [ 28].
We therefore chose to study the dynamics of rel transcription in individual cells of M. smegmatis grown in nutrient medium up to stationary phase, with nutrient depletion serving as the source of stress.
In the earlier study [ 19], we investigated the dynamics of rel transcription in individual cells of M. smegmatis grown in nutrient medium up to the stationary phase, with nutrient depletion serving as the source of stress.
This may be explained as follows: E. coli catabolised the initial nutrient supply, proliferated rapidly, and then remained in the stationary phase after nutrient depletion.
Curdlan production was not seen during the cell growth phase and nutrient limitation was required for initiation of curdlan biosynthesis (Lee at al.1997& Jung et al.2001).2001
During this phase, under nutrient-replete and light (shade -limiting conditionshade -limitingg algal biomass oconditionsurrent with andecreasincreasing ralgalculture quality) that is historically thought to generate a peak of maximum productivity.
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