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This covariance leaves potential for antagonistic pleiotropic effects among traits and repulsion phase linkages among loci.
Finally, this method uses coupling and repulsion phase linkages between loci rather than simply the repulsion allele within a locus.
The beneficial QTL alleles can be useful for tomato breeding if progeny without unfavorable repulsion phase linkages are obtained via recombination.
Among 469 linked loci on the framework map, 241 coupling vs. 228 repulsion phase linkages were detected that conformed to a 1 1 ratio, confirming disomic inheritance.
Additionally, in populations that have higher numbers of recombination between genotypes, it is expected that repulsion and coupling phase linkages will be broken, allowing for greater power to detect QTL.
If unfavorable repulsion phase linkages of QTL are broken through recombination and recombinants are recovered, then the beneficial QTL alleles we have identified may prove useful for breeding tomato cultivars.
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In view of the strong S phase linkage of I-SceI-induced HR in this system, treatments that strongly perturb HR without significantly affecting cell cycle distribution of the culture will likely affect HR during S phase.
Identification of specific allele variants contributing to improved resistance may be useful to breeders by allowing selection of resistance alleles in coupling phase linkage with favorable agronomic characteristics.
If the opposing allelic effects are caused by tightly linked genes with the favorable resistance alleles in repulsion phase linkage, a recombination event to separate the causal genes would be required to create a suitable donor parent line in coupling phase linkage for breeding purposes.
Tightly-linked QTL can be optimal for breeding if the desirable resistance alleles are in coupling phase linkage and there is no detrimental linkage drag associated with the chromosomal region.
The second phase (linkage population) will link the survey data to consultation data, for patients who will give written consent to access their clinical records from general practice in the baseline questionnaire.
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