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For these tests, cells were first grown to exponential phase in medium containing d-glucose.
The shape of the M6C area is similar to that of the Laves phase in medium alloy steels.
Consequently, in subsequent experiments the osmotic stress was applied once the cells had reached late exponential growth phase in medium with no salt.
It was observed that the yeast strains produced protein in response to Hg toxicity, particularly during lag growth phase in medium supplemented with ≥16 μg ml−1 Hg2+.
This dual role of floridoside could be responsible for the dramatic differences in glycoside content between cells growing under osmotic stress and cells stressed only after reaching late exponential phase in medium with no salt.
Growth of the final adapted strain, designated DST160, proceeded growth rate of 0.20 h− 1 without a lag phase in medium containing 0.592 M succinate, while the wild-type strain showed 0.02 h− 1 in 38 h.
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First, E. coli cells were grown to late exponential phase in M63 medium (glucose minimal medium).
Bacteria were grown under four different conditions: log phase in LB medium, stationary phase in LB medium, MgM dilution (AMM1), and MgM medium shock (AMM2) as described previously [ 24].
For the gene expression study, the planktonic cells were grown up to log phase in corresponding medium, that is, in YE medium alone or in presence of either 0.2% HY or Glu, and immediately processed for RNA extraction.
Isogenic strains were grown to mid-log phase in YNB medium, washed twice with and inoculated in starvation medium at 0.6 OD60.6m/ml.
The cells were cultivated in a 3.6-L Infors HT-Labfors bioreactor in two stages, an initial batch phase in minimal medium, followed by a fed-batch phase of adaptation in a medium of liquid fraction with minimal medium.
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