IaaS is the equivalent of every individual company getting their raw electrical power from the grid … but stepping it down with their own transformers and converting from three-phase to one-phase in-house.
Resulting tryptic peptides were desalted in C18 reverse-phase in-house ziptip columns (POROS R2 50, Applied Biosystems), as described by Jurado et al. [24].
In the first phase, an in-house implemented algorithm operates on the basis of a well-defined clustering objective.
Once this further phase of in-house testing was complete and we had implemented the final defect fixes, we released BWA v1 for general use.
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In each injection, peptides were loaded onto a 75-μm diameter and 10.5-cm-long fused silica microcapillary reverse-phase column, in-house packed with Magic C18 AQ material (200 Å pore, 5 μm diameter; Michrom BioResources).
All potential human case-patients are tested for WNV immunoglobulin M (IgM) and IgG by using ELISA FOCUS Technologiess, Cypress, CA, USA) and acute-phase and convalescent-phase serum samples; in-house hemagglutination inhibition (HI) tests are conducted when needed (16 ).
Peptides were eluted from the cartridge by application of a gradient from 0 to 90% acetonitrile in 0.1% formic acid over 40 min at 100 nl/min, and separated by passing through a C-18 reversed phase column (packed in-house with 5 µm particle size packing material from Column Engineering, Ontario, CA).
After flushing from the trapping column, the sample was loaded on a reverse-phase column (made in-house, 75 µm ID × 150 mm, 5 µm C18 Reprosil-HD beads; Dr. Maisch).
The sample was loaded on a reverse-phase column (made in-house, 75 µm I.D. × 300 mm, 1.9 µm beads C18 Reprosil-Pur, Dr Maisch GmbH, Germany) placed in a column oven (Sonation GmbH, Biberach, Germany) maintaining a constant temperature of 55°C.
Four CBCT image sets with different respiratory phases were reconstructed using in-house software, where respiratory phases were extracted from the projection data.
