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Liquid chromatographic resolution of all analytes was achieved using a 2 × 60 mm MultoHigh C18 reversed phase column with 3 μm particle size (CS-Chromatography Service).
DA was separated on a C18 reversed phase column (BDS Hypersil, 3 μm, 1 × 100 mm; Keystone Scientific, Bellefonte, PA) at 35°C.
Reversed phase column.
Gilson high-performance liquid chromatography (HPLC) was used for the semipreparative reverse phase column chromatography.
An Alltech reversed phase column (mod. Alltima C8 250 x 4.6 mm I.D).
Compounds were separated by using C18 reverse phase column (25 × 4.6 mm, particle size 5 μm).
Similar(25)
reversed-phase column using deuterated analogues of the analytes as internal standard.
The system was equipped with a reverse-phase column (3-μm particles, ALB-215 C18, 1 × 150 mm, Antec) at a flow rate of 200 μl/min.
Subsequently, this material coeluted with synthetic LHRH on a reversed-phase column as a single peak.
This IR substance coeluted with synthetic rat GAP1-56 on a reversed-phase column as a single peak.
Normal-phase column chromatography was carried out on regular silica gel 60 (0.040–0.063, Merck).
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