Sentence examples for phase buffer from inspiring English sources
Due to complexity of retention mechanisms in HILIC system, detailed examination of experimental space assessing the influence of important factors (acetonitrile content in the mobile phase, buffer concentration and pH of the mobile phase) and their interactions was done by applying 33 experimental design.
The robustness of the adopted method was demonstrated by the consistency of the values of the peak area ratios with the deliberately minor changes in the experimental parameters such as, Britton Robinson buffer of pH 4-4.5 and (0.015 M - 0.05 M) molar strength, and organic phase: buffer ratio (65 35-70 30) v/v which did not greatly affect the peak area ratios.
Samples that were not immunodepleted were diluted 1∶100 in mobile phase buffer (0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.8).
RIPA Buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 0.1% SDS, 1% Nonidet P-40) was used as the mobile phase buffer.
The mobile phase buffer was a 20 mM potassium phosphate buffer pH 7.0.
The pH of the mobile phase buffer influences the retention of nucleotides also in the case of SG-AP.
In the second stage the buffers yielding the best sensitivity and selectivity in the ionisation screening stage are used as mobile phase buffers to LC column screening with different stationary phases applying a shallow gradient.
It was observed in the case of all pH of mobile phase buffers.
Utilization of lower concentrations of mobile phase buffers caused very asymmetric peak shapes (fAS between 1.6 and 3.3).
The following mobile phase buffers were used for separation of carbohydrates: Buffer A, 15 mM NaOH, and Buffer B, 50 mM NaOH/1 M sodium acetate.
In brief, carbohydrates were separated on a CarboPac PA10 column (250 × 4 mm) with an amino-trap (50 × 4 mm) placed before the analysis column and a borate-trap (50 × 4 mm) placed before the injector port to remove trace amounts of borate from the mobile phase buffers.
