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The upper layer (polar phase; about 0.7 mL) and the lower layer (lipophilic phase; about 0.4 mL) were each dried under a stream of nitrogen (N2) and then dissolved in water (50 μL) or chloroform/methanol (2 1, v/v; 50 μL), respectively [25],[25].
The parasites were harvested at early stage of stationary phase (about 120 h later), washed with PBS (pH = 7.2), and subjected to trypsin digestion.
Analyses of samples withdrawn at the time points for transition from exponential to stationary growth phase (about 72 hours) showed a similar activity for the LU-5 promoter variant and the wild-type promoter, whereas variant LU-4 displayed a 2-fold increase in luciferase activity compared to that of the wild-type (data not shown).
All the cultures were incubated until they reached stationary phase (about 109 cells per ml).
Drawing machine in first phase about to be engaged in action.
He also testified during the sentencing phase about how the loss of his father had devastated his family.
In the double-beta phase about 0.3% natural tellurium will be loaded in the 780 tonnes of liquid scintillator.
Information from the design phase about construction components and scheduling has been gathered to formulate a 4 dimensional (4D) model.
China kicked off its foreign ownership phase about a decade ago when it started buying US treasuries, the debt issued by the American government.
Here the hydrothermal medium is a water-rich phase about 200C at sufficiently high pressures to keep the water in either a liquid or supercritical state.
The as-sprayed NiCrBSi coating possesses a mixed microstructure mainly composed of the crystalline phases and the amorphous phase (about 10 vol%).
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