ELISA diagnostic kits for HBV serologic markers, anti-HAV, anti-HCV, and anti-HEV IgG were all purchased from Beijing Wantai Biological Pharmacy Enterprise Co., Ltd.
HIV status test was carried out using an enzyme-linked immunosorbent assay (ELISA, Wantai Biological Pharmacy Enterprise Co., Ltd, Beijing, China) and confirmed by another ELISA kit (Shanghai Kehua Bio-engineering Co., Ltd., Shanghai, China).
The levels of IgM or IgG antibody against HEV were determined by commercial enzyme-linked immunosorbant assays (ELISA) (Beijing Wantai Biological Pharmacy Enterprise Co., Ltd., Beijing, China) according to the manufacturer's instructions.
All specimens were also tested for IgM antibody against hepatitis A virus (HAV-IgM), IgM antibody against hepatitis B core protein (HBc-IgM), hepatitis B virus surface antigen (HBsAg), and antibody against hepatitis C virus (HCV-Ab) using commercial ELISA kits (Beijing Wantai Biological Pharmacy Enterprise Co., Ltd., Beijing, China).
3, 5 Over the past few years, Beijing Wantai Pharmacy Enterprise Co., Ltd.
HBsAg was tested using an ELISA kit (Wantai Biotech Pharmacy Enterprise Co. Beijing, China).
For diagnosis, an enzyme-linked immunosorbent assay for HEV IgG and IgM (Beijing Wantai Biological Pharmacy Enterprise CO., LTD).
All samples were tested for HEV IgG by using the Wantai HEV-IgG ELISA (Beijing Wantai Biologic Pharmacy Enterprise Co., Ltd, Beijing, China).
Anti-HIV IgG antibody and HBV surface antigen (HBsAg) were tested using an ELISA technique (Wantai Biological Pharmacy Enterprise Co., Beijing, China).
IgG and IgM against HEV were detected by using an enzyme immunoassay (Wantai Biologic Pharmacy Enterprise, Beijing, China) according to the manufacturer's instructions (13 ).
