Circulating levels of human GLP-1 and GIP were determined by enzyme-linked immunosorbent assay (ELISA) using the GLP-1 (7-36) amide/prepro-glucagon (98-127) amidenzymeme immuno assay EIA kit (Phoenix Pharmaceuticals, Inc., Burlingame, CA) and the human GIP assay kit (Immuno-Biological LaboraTakasakio., Ltd., Takasaki, Japan), respectively.
Products included salt tablets, water purification tables, insect repellents, pharmaceuticals, paratrooper kits and gas mask canisters.
A commercial guaiacol detection kit (Kyokuto Pharmaceutical Co., Ltd., Japan) was used to confirm the presence of Alicyclobacillus in these colonies.
Immunohistochemical reactions were visualized using a HistoFine kit (Nichirei Pharmaceutical, Tokyo, Japan) and a DAB kit (Dako, Carpinteria, CA, USA).
All patients underwent urine antibody testing (Rapirun® H. pylori Antibody Detection Kit, Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan).
Blood glucose was measured via an enzyme electrode method using a Free Style kit (Kissei Pharmaceutical, Nagano, Japan).
Blood glucose was measured by the enzyme electrode method using a Free Style kit (Kissei Pharmaceutical, Nagano, Japan).
Third, from this collected saliva, cotinine levels were then determined using a NicAlert Kit (Nymox Pharmaceutical Corp., Maywood, NJ).
Serum high-sensitivity C-reactive protein (hs-CRP) was measured using a CRP-Latex kit (Nissui Pharmaceutical Co. Ltd., Tokyo, Japan) according to the manufacturer's instructions.
We evaluated samples using the Wantai (PE2) HEV IgG ELISA kit (Wantai Pharmaceutical Enterprise, Beijing, China), which detects IgG for all 4 known strains of human HEV.
HBV DNA level was determined by a real-time polymerase chain reaction (PCR) kit (Fosun Pharmaceutical Co., Shanghai, China) with a lower limit of detection of 500 copies/mL (about 100 IU/mL).
