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Based on the consumption of nutrients in the medium observed during batch culture, a fed-batch fermentation strategy with controlled DO and pH was implemented.
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For example, a fast response optical pH sensor was implemented on this principle.
Continuous pH monitoring was implemented within the 1.5-L loop air-lift photobioreactors supplemented with 5% CO2 (v/v) in air.
If blood gas analysis was implemented, pH ≤ 7.2, base excess ≤ -5 mmol/l and PaCO2 ≥ 50 mmHg defined a severe metabolic response.
The authors of the manuscript (NK, GJ, PH) developed the search strategy and it was implemented by NK.
This method was implemented based on the use of pH indicators because of the changes in pH during the fermentation process.
Afterwards, investigation of the effects of temperature and pH on the activities expressed by the different clones was implemented.
The assay was implemented for simultaneous analysis of Akt-PH domain, indicating PI 3,4,5 P3 and PI 3,4 P2 as the primary ligands.
This, allowed manual base only pH control to be implemented in microwell fermentations which enhanced both the maximum specific growth rate and the maximum biomass concentration.
Using the HMF concentration in the control run as the inhibitor tolerance limit, an estimated solid loading of approximately 38% can be implemented using pH profiling pretreated whole slurry of FS-10 without detoxification.
Then, the overall proposed control structure is implemented in a pH neutralization experimental setup.
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