Sentence examples for ph gel from inspiring English sources
Through control of pH, gel stoichiometry, and crosslinker structure, viscoelastic properties in these crosslinked networks can be modulated across several orders of magnitude.
In a preliminary experiment, we used an immobilized linear pH gel strip with a broad pH range (pH 3-10 linear) for 1D IEF.
Therefore, we performed subsequent analyses using an immobilized linear pH gel strip with a narrower range (pH 4-7 linear) to obtain a finer resolution.
Non-equilibrium pH gel electrophoresis (NEPHGE) technique was developed to resolve proteins with basic to extremely high pI (7.0 to 11.0) [ 1, 44] that cannot be separated by the traditional method.
The sample was pipetted into an 11 cm Ettan IPGphor strip holder (Amersham Biosciences) and overlaid with an 11 cm Immobiline Drystrip 4-7 pH gel strip (GE Biosciences) with the backing strip of the gel toward the top.
For each age-group, 3 samples were labeled with Cy3 and 3 samples with Cy5, with the samples then randomized to 42 gels run during this project (21 gels for pH 3 11; 21 gels for pH 4 7).
These gels were characterized by pH, viscosity, gel strength and FTIR study.
SDS-PAGE was carried out in 1X SDS running buffer (10X Tris-Glycine-SDS buffer, BioRad, Hercules, CA, USA) using a constant current of 15 mA for the stacking (4.2% acrylamide, pH 6.8) gel and then a constant 25 30 mA for the separating (15% or 18% acrylamide, pH 8.8) gel in a Mini-PROTEAN 3 electrophoresis apparatus (BioRad), according to the Laemmli method.
The buffer conductivities are measured as 1.3 mS/cm for the Tris tricine run buffer, 0.47 mS/cm for the Tris glycine run buffer, 5.1 mS/cm for the 500 mM Tris HCl pH 8.45 gel buffer in the Tris tricine system, and 3.1 mS/cm for the 375 mM Tris HCl pH 8.8 gel buffer in the Tris glycine system.
The pH values, gel times and water uptakes were considered as responses (dependent variables).
