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Unlike the PG tree, the algal root is a member of one of the polytomies.
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In the observed population of Platycarya, 25 PA and 22 PG trees were observed (of these, temporal sex expression was monitored for 19 PA and 13 PG trees).
In 2010, 72 male spikes at stage M1 were taken from two PA and two PG trees.
The temporal changes in sex expression of 19 PA and 13 PG trees were typical of heterodichogamous populations (Fig. 4).
Within each tree, the M1 male phase tended to overlap with the preceding F1 female stage, while M2 was temporally distinct, except for several PG trees in 2009.
In 2009 and 2010, spikes of PA and PG trees at various stages were collected to count thrips (see Table 4 for the number of spikes).
Of the 12 PG trees that were monoecious in 2008, six were also monoecious in 2009, and six did not flower in 2009.
In the 22 PG trees, inflorescences were composed of a central androgynous spike (a basal female portion and an apical male portion) and up to ten lateral male spikes (Fig. 1, PG).
Only 13 (seven PA, six PG) trees set monoecious inflorescences every year, and the others either flowered as functional males or not at all in 1 or 2 years.
Like the PGs, the PME gene tree we generated has two large polytomies and two smaller resolved clades.
The midpoint-rooted maximum likelihood phylogenetic tree shows PGs, with tips and terminal branches collared by continent of origin.
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