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Figure 12 The results of the improved PFA in Figure5.
to compare maternal fetal attachment (MFA) and paternal fetal attachment (PFA) in terms of selected variables.
Then the embryos were fixed with 1% PFA in PBS in 4°C overnight.
The efficiency of PFA in MTRC compensation will be also illustrated.
4% PFA in PBS was immediately applied for 10 minutes.
Cells were fixed in 4% PFA in PBS.
Eyes were placed in 4% PFA in PB overnight.
Samples were fixed with 4% paraformaldehyde (PFA) in relaxing solution.
After treatment the cells were washed with PBS and fixed with 4% PFA in PBS.
Adherent cells were fixed with 3.7% PFA in PBS and then stained with Coomassie blue.
Brains were removed and post-fixed overnight with 4% PFA in 30% sucrose.
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