Sentence examples for pets containing the from inspiring English sources

Salmonella infections can originate from household pets containing the bacteria, particularly reptiles, improperly prepared meats and seafood, or the surfaces of raw eggs, fruits, or vegetables that have not been adequately disinfected.

Bacteria were transformed with a plasmid (pET) containing the open reading frame of wild-type mouse PrP (amino acid residues 23 231) or mutants of this construct.

Using plasmid DNA (pET 100) containing the 211 bp target WSSV sequence, analytical sensitivity showed that the 2-step nested PCR protocol was able to detect down to 0.015 fg of the plasmid DNA, or approximately 2 copies of the target DNA sequence.

Plasmid pET-28a containing the FMDV 3Dpol coding sequence with an AAALE linker at the carboxyl terminus followed by 6 histidines was obtained from Drs. Verdaguer and Domingo [32].

His-tag expression vectors (pET-16b) containing the wild-type and a cysteine-less mutant of KCNE1 were transformed into XL1-Blue Escherichia coli cells (Stratagene).

The MalE-pET vectors containing the PARP-1 constructs were transformed into Escherichia coli Rosseta2 BL21(DE3) (Novagen) and grown at 37 °C in 6 L of Luria-Bertani broth containing 50 μg/mL kanamycin.

Of the above E. coli strains, the highest activity of approx. 230 U L-1 was obtained in BL21-Gold DE3) strain carrying the pOK101 plasmid (pET-30 containing the nit gene).

E. coli BL21 DE3) RIPL cells (Stratagene) were transformed with pET vectors containing the S. cerevisiae gene of interest and induced at OD600 0.6 with 1 mM IPTG at 23 °C over night.

ELPs were expressed from pET-25b-1 conthening the [V720 or [RGD-V620 gene in 40 l culture of E. coli BLR DE3) (Novagen). Protein expression was induced at an OD600 of about 0.6 with 1 mM β-isopropyl thiogalactoside (Sigma) and allowed to express for 4 h post induction. The ELPs were by phase transition cycling [ 19].

However, this increase appeared not to be the function of enzymes, as similar increases of growth were also seen in the strains transformed with pET-RBC197-PRK containing the deactivated PRK (Fig.  2b) and pET-RBC197-PRK2021 containing both deactivated enzymes (Additional file 1: Figure S3).

E. coli BL21 (DE3) containing pASZ2 (pET 11a derivative containing the gene for TADH) was grown in Overnight Express TM Autoinduction System (Novagene) at 37°C for 24 h.