In 1997 Strobel filled a box with petri dish cultures of endophytes collected on a tromp through a Honduran jungle.
Bill Gates once told me that The Forbes 400 issue is "capitalism in a box, a petri dish of success and failure".
A liquid nitrogen bath was prepared by inserting a 60 mm (D ×15 mm (H) Petri dish inside a Styrofoam box, filling the dish and surrounding box area with liquid nitrogen, and placing the box 20 mm underneath the valve.
With the Bremen defence backtracking, Bale delivered the perfect cut-back, skidding a low centre towards the six-yard box that left Petri Pasanen, who had Crouch breathing down his neck, with no option but to make contact; he turned the ball past his own goalkeeper.
Drops were maintained on sterile Petri dishes placed in humid boxes at 30° and 28° for T. thermophila and T. pyriformis, respectively, which are the temperatures normally used in laboratory to grow cultures of each species.
Evaporation was prevented by placing the Petri dishes in a humidified metal box and the medium was carefully exchanged every 24 hrs with fresh, prewarmed medium to ensure nutrient and oxygen availability.
The cells were then immediately transferred into Hungate tubes with 5 ml fresh RCM medium, incubated at 45°C for 5 h, diluted and mixed with melted warm RCM-agar medium supplemented with 250 µg/ml kanamycin, and then plated onto Petri dishes inside an anaerobic glove box.
To avoid any potential atmospheric exchange of volatiles that could have interfered with our measurements, each experimental unit was transferred one at a time to a separate room where the 2 external square boxes were opened; all petri dishes were then removed and inspected, while the rest of the unit (including the base and the central cylindrical box) was taken outdoors and opened.
Sterile culturing of SAM-less monocot seedlings was initially performed on 0.5X MS in petri dishes and later in magenta boxes respectively under continuous light and 21 °C.
Petri-dishes were put in a cooler box and transported to the laboratory in Blantyre for processing.
Each day we cleaned the spawning boxes, placed fertilized eggs on petri dishes, and transferred the petri dishes to an incubator (Tintometer GmbH; T = 26°C).
