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The fluorescence spectra and theoretical calculation of the dyads 1 3 revealed the photoinduced electron transfer (PET) interaction existed in the dyads in their excited state.
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Pet interactions are stimulated through interaction with human users or between other pets within the population.
A ChIA-PET interaction region may contain a cCTCF site, a non-constitutive CTCF site, or neither.
We then counted the number of cCTCF and non-constitutive CTCF sites contained within any regions in the ChIA-PET interaction data.
The proportion of ChIA-PET interaction sites among these cCTCF sites was significantly higher than the corresponding proportion among the non-constitutive CTCF binding sites (odds ratio=7.7, p-value≈0, one-sided Fisher exact test) (Table 3a).
Indeed, using ChIA-PET interaction information [ 15], we found that those regions that gained connections to their target genes were significantly upregulated during differentiation compared with the target genes in other clusters.
Thus, a ChIA-PET interaction pair may be one of six possible types: cCTCF and cCTCF, cCTCF and non-constitutive CTCF, cCTCF and neither, non-constitutive CTCF and non-constitutive CTCF, non-constitutive CTCF and neither, neither and neither (Table 4).
ChIA-PET interactions were additionally filtered to remove interactions between regions ≤1000 bp apart.
We confirm the ChIA-PET interactions using standard 3C assays in brain.
We found <4% (352 out of 8817) of the sorted GWAS regions had more ChIA-PET interactions than the SLC2A9 region (i.e. chr4: 10080000–10105000, with 96 ChIA-PET interactions).
Including these additional sites in our analysis of ChIA-PET interactions yielded results substantially the same as those in Table 4.
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