However, the biological environment of proteins can differ from these in vitro conditions in ways that could significantly perturb stability.
Thus, the introduction of mutations into the CaM binding site led to a faster exchange (in the complementation assay), meaning that those mutations perturbed the stability of the tetrameric assembly.
Changing foot placement perturbs stability through redistributing body mass and deviating COG locations in the dimensions.
eIGPS-Δβ1α1-S82A and eIGPS-Δβ3α3-D132A have no significant effect on the stability of N, and none of the clamp-deletion variants perturb the stability of I relative to U (Figure 3D and Table 1).
It significantly reduced NPM1 expression and, in synergy but independently of TP53 deletion and/or mutation, was associated with a strong tendency to perturb chromosomal stability.
The authors suggested that the presence of multiple microtubule asters might perturb the stability of kinetochore attachments [26], thus leading to an increase in the time necessary to complete kinetochore attachment, and consequently lengthening mitosis.
Despite initial considerations, most pathogenic mutations rarely alter the native structure of PrPC, but perturb its stability, regulate its metabolism, modulate the oligomerization pathways and determine the features of the PrPSc assemblies [2],[18] [27][18]–[27].
Together, these reports suggest that the presence of an isolated dxN may perturb the stability and structure of duplex DNA in vivo.
The 14 sequence variants associated with low NPC1L1 levels were distributed throughout the protein, and the mechanism(s) by which they perturb protein stability is not known.
Achieving titratable control in these organisms is more challenging because of limited genetic tools as well as growth conditions that perturb the stability and function of the inducers or regulators.
