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For example, the sum of cofactors and their intermediates in the new perturbed condition must be equal to those in reference steady state.
In all, 67% of the metabolic signatures are unique with most of the remaining similar to only one other perturbed condition (Table 2).
The cell cycle progression phenotype is quantified based on the percentage of the cells in a cell cycle state in perturbed condition with respect to the unperturbed condition.
In this framework, metabolic flux data obtained from wild-type (control condition) and its genetic mutant (perturbed condition) were treated together (see step C below for the reason).
Simulation of the metabolic network model along with data preprocessing were implemented in GAMS for the wild-type (control condition) and lpdA mutant (perturbed condition), separately.
In this study, a total of 2,000 samples were generated at the end, each 1,000 sets of data from the wild-type (control condition) and its genetic mutant (perturbed condition), respectively.
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The rest of the intervals, with increments of 500 units, cover perturbed conditions.
Further, a Monte Carlo analysis is performed to examine the performance of the OSG law under perturbed conditions.
Numerical simulations are conducted under both nominal and perturbed conditions to demonstrate robustness of the control design.
Also, the experiments confirm that the proposed controller is capable to tolerate the perturbed conditions and can be alternative to the conventional controllers in pneumatic controlled system.
The effectiveness of the proposed control scheme is validated through simulations on a 4-cable planar robot in both nominal and perturbed conditions.
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