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A robust signal was observed at 3 dpa, and then the expression persisted in the blastema of regenerating fins during regenerative outgrowth.
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Indeed, expression of runx2 and osterix persisted in the proximal blastema of MGCD0103-treated findicatingathat that blastema cells were blocked in an intermediate state.
In stark contrast to development, ARF GFP was induced and highly expressed in the blastema of regenerating adult fins.
In samples from the side of the head opposite to the blastema of regenerates, differentially-expressed genes again fell into diverse functional categories.
GFP expression is induced in the proliferative blastema of the regenerate, but it is not expressed in the surrounding epithelium.
The gene for this protein was upregulated in the established blastema of the regenerating zebrafish fin [ 104].
The vast majority of neoblast-expressed genes were also expressed in the blastema (127 out of 139 genes; Fig. 2g), and many were found among the genes identified by microarray to be upregulated in the blastema (Fig. 2d).
With the exception of the dpy30 homologs, each of these genes is upregulated in the blastema during the earliest days of regeneration (day 1 3; Fig. S4 ).
Intriguingly, pou5f1 was not completely shut down in adult fins and was also expressed in the blastema, while no expression was found in blastemas of either newts or Xenopus ([ 12], this paper).
Quantification of proliferating cells (Ki67+) in WT blastema and non-blastema components revealed significantly higher numbers of Ki-67+ cells in the blastema.
At the early bud stage, when blastema cells are proliferating rapidly, a strong expression of TGF-β1 is present in the blastema.
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