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Using the special kits (peroxide test) for measuring H2O2 (hydrogen peroxide) through the inserted directives on it and comparing with color change, the target numbers were obtained.
At baseline, discharge, and at 3, 6, 9, and 12 months' corrected age, we assessed anthropometry; infections; red blood cell hemoglobin, catalase, glutathione peroxidase, red blood cell fragility (hydrogen peroxide test), and superoxide dismutase values; plasma malondialdehyde, ferritin, iron, transferrin, zinc and copper levels; and diet intake.
After 3 h no peroxides were detectable (peroxide test strip), which was confirmed the next morning.
overnight (no peroxides were detectable with peroxide test strips after 3 h).
Hydroperoxide tests used Quantofix peroxide test strips (Sigma-Aldrich, Inc., Milwaukee, WI) that have a sensitivity reported to detect a lower limit of 1 mg/L peroxide.
These observations are consistent with the presence of an organic hydroperoxide in MEU; this was confirmed by peroxide test strips that provided a semiquantitative estimate of 10 50 mg/L peroxide concentration.
Similar(53)
All peroxides tested revealed an inhibitory effect on P. olseni proliferation at micromolar concentrations.
H2O2 analysis was performed using peroxide colorimetric test strips (Mquant peroxide colorimetric test strips, Merck Millipore).
As an inter-assay standard the action of hydrogen peroxide was tested in every test plate.
At 25 h pH was measured using a Mettler Toledo 'SevenEasy' pH meter, and H2O2 assayed by pipetting 50 μL of medium on to Quantofix Peroxide 100 test strips and recording the colour change as per the manufacturer's instructions (Macherey-Nagel GermanyCo KGermanyany).
The hydrogen peroxide concentration of each test dilution was determined one hour after dilution using Peroxid-Test strips (Merck).
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