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In our implementation, this is done using at most 10 000 independent permutations of genes (fewer if the estimated P-value > 0.01).
Because the numbers of differentially expressed homologs were significantly lower than in macaque (Additional File 5) we examined the significance of the performance value for each cluster relative to 25 random permutations of genes for that cluster.
Other gene set combinations were taken from the remaining permutations of genes generated from those with at least 2 RMP orthologs as well as the rodent parasite specific genes.
TRDV1 subgroup members are found on 4 different homology units (homology units 7, 8, 9 and 10), intercalated with different permutations of genes including members of the TRAV22, 23, 25 and 26 subgroups.
Notably, TRDV1 genes are co-located in 4 different homology units with different permutations of genes from 4 of the other massively expanded subgroups - TRAV22, 23, 25 and 26.
The expression of three of these 'bins' (gene sets partitioned by binding frequency) was significantly enriched in the control group (i.e., repressed in the seizure group) when compared to the levels of enrichment of gene sets composed of random permutations of genes (FWER *p < 0.05).
Similar(54)
The permutation analysis (see M&M) was based on 1000 permutations of gene-day signals across all 22000 genes at 5 days.
Since randomly ordered data should destroy correlations, we expect I to be close to zero, therefore, we calculated the minimum I for 100 random permutations of gene deviation vectors { V ti)}.
The significance of the enrichment scores was tested against 10,000 permutations of gene names.
Each set of composite motifs has a q-value derived from 1000 random permutations of gene labels.
One hundred permutations of gene order in WGAS ranks were examined for possible asymmetries obtained by chance.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com